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Email: ajg60@cam.ac.uk

Dr Andrew Grant

Fellow, Director of Studies in Veterinary Sciences
University Positions
University Associate Professor in Microbial Pathogenesis
Subjects
Veterinary Medicine
Specialising in
Functional genomics and genomics of bacterial pathogens

Andrew Grant is a University Associate Professor in the Department of Veterinary Medicine and a Fellow of Jesus College, Cambridge.

Academic interests

Andrew has an interest in bacterial pathogens of veterinary and clinical importance both in the UK and overseas. His research uses genomic and functional genomic approaches, combined with advanced molecular, proteomic and microscopy techniques, and often integrated with mathematical and statistical models, to determine the basis by which bacterial pathogens survive in the environment, colonise their hosts and cause disease, and to use this knowledge to develop and test novel therapeutic approaches.

Degrees obtained

  • BSc in Biochemistry and Pharmacology, University of Southampton (1997).
  • PhD, University of Southampton (2001).

Other interests

Family, cooking, food and sport.

Department link

https://www.vet.cam.ac.uk/

Publications, links and resources

  • Kunadu AP-H, Holmes M, Miller EL, Grant AJ. (2018) Microbiological quality and antimicrobial resistance characterization of Salmonella spp. in fresh milk value chains in Ghana. Int. J. Food. Microbiol. 277: 41-49.
  • de Vries SPW, Vurayai M, Holmes M, Gupta S, Bateman M, Goldfarb D, Maskell DJ, Matsheka MI, Grant AJ#. (2018) Phylogenetic analyses and antimicrobial resistance profiles of Campylobacter spp. from diarrhoeal patients and chickens in Botswana. PLoS One. 13(3): e0194481.
  • de Vries SPW, Hadjirin NF, Lay EM, Zadocks RN, Peacock AJ, Parkhill J, Grant AJ, McDougall S, Holmes MA. (2018) Streptococcus bovimastidis sp. Nov., isolated from a dairy cow with mastitis. Int. J. Syst. Evol. Microbiol. 68(1): 21-27.
  • Bateman M, de Vries SPW, Gupta S, Guardabassi L, Cavaco L, Grant AJ, Holmes MA. (2017) Genome and plasmid sequences of Escherichia coli KV7, an extended-spectrum b-lactamase isolate derived from faeces of a healthy pig. Genome. Announc. 5(38). Pii: e00595-17.
  • Rossi O, Dybowski R, Maskell D, Grant AJ, Restif O, Mastroeni P. (2017) Within-host spatiotemporal dynamics of Salmonella infection during and after antimicrobial treatment. J. Antimicrob. Chemother. 72(12): 3390-3397
  • Oshota O, Conway M, Fookes M, Schreiber F, Chaudhuri RR, Yu L, Morgan FJE, Clare S, Choudhary J, Thomson NR, Lio P, Maskell DJ, Mastroeni P, Grant AJ#. (2017) Transcriptome and proteome analysis of Salmonella enterica serovar Typhimurium systemic infection of wild type and immune-deficient mice. PLoS One. 12(8):e0181365
  • de Vries SPW, Gupta S, Baig A, Wright E, Wedley A, Jensen AN, Lora LL, Humphrey S, Skovgard H, Macleod K, Pont E, Wolanska DP, L’Heureux J, Mobegi FM, Smith DGE, Everest P, Zomer A, Williams N, Wigley P, Humphrey T, Maskell DJ, Grant AJ#. (2017) Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models. Sci. Rep. 7: 1251.
  • Esson D, Gupta S, Bailey D, Wigley P, Wedley A, Mather AE, Meric G, Mastroeni P, Sheppard SK, Thomson NR, Parkhill J, Maskell DJ, Christie G, Grant AJ#. (2017) Identification and initial characterisation of a protein involved in Campylobacter jejuni cell shape. Microb. Pathog. 104: 202-211.
  • de Vries SP, Linn A, Macleod K, MacCallum A, Hardy SP, Douce G, Watson W, Dagleish MP, Thompson H, Stevenson A, Kennedy D, Baig A, Coward C, Maskell DJ, Smith DGE, Grant AJ+#, Everest P+. (2017) Analysis of Campylobacter jejuni infection in the gnotobiotic piglet and genome-wide identification of bacterial factors required for infection. Sci. Rep. 7: 44283.
  • Rossi O, Grant AJ, Mastroeni P. (2017) Effect of in vivo neutralisation of tumor necrosis factor alpha on the efficacy of antibiotic treatment in systemic Salmonella enterica infections. Pathog. Dis. 75: doi: 10. 1093/femspd/ftx002.
  • Yahara K, Meric G, Murray S, Taylor AJ, de Vries SPW, Murray S, Pascoe B, Mageiros L, Torralbo A, Vidal A, Ridley A, Komukai S, Wimalarathna H, Cody A, Colles FM, McCarthy N, Harris D, Bray JE, Jolley KA, Maiden MCJ, Bentley SD, Parkhill J, Bayliss CD, Grant AJ, Maskell DJ, Didelot X, Kelly DJ, Sheppard SK. (2017) Genome-wide association of functional traits linked with Campylobacter jejuni survival from farm to fork. Environ. Microbiol. 19: 361-380.
  • Scanlan E, Yu L, Maskell D, Choudhary J, Grant AJ#. (2017) A quantitative proteomic screen of the Campylobacter jejuni flagellar-dependent secretome. J. Proteomics. 152: 181-187.
  • de Vries SP, Gupta S, Baig A, Wright E, Wedley A, Nygaard Jensen A, LaCharme Lora L, Humphrey S, Skovgard H, Macleod K, Pont E, Wolanska DP, L’Heureux J, Mobegi FM, Smith DGE, Everest P, Zomer A, Williams N, Wigley P, Humphrey T, Maskell DJ, Grant AJ#. (2016) Genome-wide fitness analyses of Campylobacter jejuni in in vitro and in vivo models. bioRxiv doi: https://doi.org/10.1101/085720
  • Esson D, Mather AE, Scanlan E, Gupta S, de Vries SP, Bailey D, Harris SR, McKInley TJ, Meric G, Berry SK, Mastroeni P, Sheppard SK, Christie G, Thomson NR, Parkhill J, Maskell DJ, Grant AJ#. (2016) Genomic variations leading to alterations in cell morphology of Campylobacter spp. Sci. Rep. 6: 38303
  • Giacomin P, Zakrzewski M, Jenkins T, Su X, Al-Hallaf R, Croese J, de Vries S, Grant A, Mitreva M, Loukas A, Krause A, Cantacessi C. (2016) Changes in duodenal tissue-associated microbiota following hookworm infection and consecutive gluten challenges in humans with coeliac disease. Sci. Rep. 6: 36797
  • Rosconi F, de Vries SP, Baig A, Fabiano E, Grant AJ#. (2016) Essential genes for in vitro growth of the endophyte Herbaspirillium seropedicae SmR1 revealed by transposon insertion sequencing. Appl. Environ. Microbiol. 82: 6664-6671.
  • Yahara K, Meric G, Murray S, Taylor AJ, de Vries SPW, Murray S, Pascoe B, Mageiros L, Torralbo A, Vidal A, Ridley A, Komukai S, Wimalarathna H, Cody A, Colles FM, McCarthy N, Harris D, Bray JE, Jolley KA, Maiden MCJ, Bentley SD, Parkhill J, Bayliss CD, Grant AJ, Maskell DJ, Didelot X, Kelly DJ, Sheppard SK. (2016) Genome-wide association of functional traits linked with Campylobacter jejuni survival from farm to fork. Peer J doi: 10.7287/peerj.preprints.2300v1
  • Grant AJ#, Oshota O, Chaudhuri RR, Mayho M, Peters SE, Clare S, Maskell DJ, Mastroeni P. (2016) Genes required for the fitness of Salmonella enterica serovar Typhimurium during infection of immunodeficicent gp91-/-phox mice. Infect. Immun. 84, 989-997.
  • Goh YS, Armour KL, Clark MR, Grant AJ, Mastroeni P. (2016) IgG subclasses targeting the flagella of Salmonella enterica serovar Typhimurium can mediate phagocytosis and bacterial killing. J. Vaccines Vaccin. 7(3), pii: 322.
  • Dybowski R, Restif O, Goupy A, Maskell DJ, Mastroeni P, Grant AJ#. (2016) Correction to ‘Single Passage in mouse organs enhances the survival and spread of Salmonella enterica’. J. Roy. Soc. Inter. 13(115):20160040.
  • Dybowski R, Restif O, Goupy A, Maskell DJ, Mastroeni P, Grant AJ#. (2015) Single Passage in mouse organs enhances the survival and spread of Salmonella enterica. J. Roy. Soc. Inter. 12 (113):20150702.
  • Giuliano A, Meiring T, Grant AJ, Watson PJ. (2015) Acute hepatic necrosis caused by Salmonella enterica serotype I 4,5,12:-:1,2 in a dog. J. Clin. Microbiol. 53: 3674-3676.
  • de Vries SP, Gupta S, Baig A, L’Heureux J, Pont E, Wolanska DP, Maskell DJ, Grant AJ#. (2015). Motility defects in Campylobacter jejuni defined gene deletion mutants caused by second site mutations. Microbiology. 161, 2316-2327.
  • Kanji A, Jones MA, Maskell DJ, Grant AJ#. (2015) Campylobacter jejuni PflB is required for motility and colonisation of the chicken gastrointestinal tract. Microb. Pathog. 89, 93-99.
  • Kaiser P, Regoes R, Dolowschiak T, Wotzka SY, Lengefeld J, Slack E, Grant AJ, Ackermann M, Hardt W-D. (2014) Cecum lymph node dendritic cells harbour slow-growing bacteria phenotypically tolerant to antibiotic treatment. PLos Biol. 12(2):e1001793.
  • Coward C, Restif O, Dybowski R, Grant AJ, Maskell DJ, Mastroeni P. (2014) The effects of vaccination and immunity on bacterial infection dynamics in vivo. PLoS Pathog. 10(9):e1004359.
  • Kaiser P, Slack E, Grant AJ, Hardt W-D, Regoes RR. (2013) Lymph node colonization dynamics after oral Salmonella Typhimurium infection in mice. PLoS Pathog. 9(9):e1003532.
  • Mastroeni P and Grant A. Dynamics of spread of Salmonella enterica in the systemic compartment. (2013) Microbes. Infect. 15, 849-857.
  • Bulmer DM, Kharraz L, Grant AJ, Dean P, Morgan FJ, Karavolos MH, Doble AC, McGhie EJ, Koronakis V, Daniel RA, Mastroeni P, Khan CMA. (2012) The bacterial cytoskeleton modulates motility, type 3 secretion and virulence in Salmonella. PLoS. Pathog. 8(1):e1002500.
  • Staniewicz L, Donald AM, Stokes DJ, Thomson N, Sivaniah E, Grant AJ, Bulmer D, Khan CMA. (2012) The application of STEM and in-situ controlled dehydration to bacterial systems using ESEM. Scanning. 34, 237-246.
  • Holt JP, Grant AJ, Coward C, Maskell DJ, Quinlan JJ. (2012) Cj1051c is a Major Determinant for the Restriction Barrier of Campylobacter jejuni Strain NCTC11168. Appl. Environ. Microbiol. 78, 7841-7848.
  • Periaswamy B, Maier L, Vishwakama V, Slack E, Kramer M, Andrews-Polymenis HL, McClelland M, Grant AJ, Suar M, Hardt W-D. (2012) Live attenuated S. Typhimurium vaccine with improved safety in immuno-compromised mice. PLoS One. 7(9):e45433.
  • Grant AJ#, Morgan FJE, McKinley TJ, Foster GL, Maskell DJ, Mastroeni P. (2012) Attenuated Salmonella Typhimurium lacking the pathogenicity island-2 type 3 secretion system grow to high bacterial numbers inside phagocytes in mice. PLoS Pathog. 8(12): e1003070.
  • Restif O, Goh YS, Palayret M, Grant AJ, McKinley TJ, Clark MR, Mastroeni P. (2012) Quantification of the effects of antibodies on the extra-and intracellular dynamics of Salmonella enterica. J. R. Soc. Interface. 10(79):20120866.
  • Mastroeni P, Morgan FJ, McKinley TJ, Shawcroft E, Clare S, Maskell DJ, Grant AJ#. (2011) Enhanced virulence of S. enterica serovar Typhimurium after passage through mice. Infect. Immun. 79, 636-643.
  • Goh YS, Grant AJ, Restif O, McKinley TJ, Armour KL, Clark MR, Mastroeni P. (2011) Human IgG isotypes and activating Fcgamma receptors in the interaction of Salmonella enterica serovar Typhimurium with phagocytic cells. Immunol. 133, 74-83.
  • Wheeler DW, Thompson AJ, Corletto F, Reckless J, Loke JCT, Lapaque N, Grant AJ, Mastroeni P, Grainger DJ, Padgett CL, O’Brien JA, Miller NG, Trowsdale J, Lummis SC, Menon DK, Beech JS. (2011) Anaesthetic impairment of immune function is mediated via GABA(A) receptors. PLoS One. 6(2):e17152.
  • Janis C, Grant AJ, McKinley TJ, Morgan FJE, John VF, Houghton J, Kingsley RA, Dougan G, Mastroeni P. (2011) In vivo regulation of the Vi antigen in Salmonella and induction of immune responses with an in vivo inducible promoter. Infect. Immun. 79, 2481-2488.
  • Mastroeni P, Grant AJ. (2011) Spread of Salmonella enterica in the body during systemic infection: unravelling host and pathogen determinants. Expert Rev. Mol. Med. 13:e12.
  • Jansen AM, Hall LJ, Clare S, Goulding D, Holt K, Grant AJ, Mastroeni P, Dougan G, Kingsley RA. (2011) A Salmonella Typhimurium-Typhi genomic chimera: A model to study Vi polysaccharide capsule function in vivo. PLoS Pathog. 7(7):e1002131.
  • Chaudhuri RR, Yu L, Kanji A, Perkins TT, Gardner PP, Choudhary J, Maskell DJ, Grant AJ#. (2011) Quantitative RNA-seq analysis of the Campylobacter jejuni transcriptome. Microbiol. 157, 2922-2932.
  • Buckley AM, Wang J, Hudson DL, Grant AJ, Jones MA, Maskell DJ, Stevens MP. (2010) Evaluation of live-attenuated Salmonella vaccines expressing Campylobacter antigens for control of C. jejuni in poultry. Vaccine. 28, 1094-1105.
  • Javed MA, Grant AJ, Bagnall MC, Maskell DJ, Newell DG, Manning G. (2010) Transposon mutagenesis in a hyper-invasive clinical isolate of Campylobacter jejuni reveals a number of genes with potential roles in invasion. Microbiol. 156, 1134-1143.
  • Osman D, Waldron KJ, Denton H, Taylor CM, Grant AJ, Mastroeni P, Robinson NJ, Cavet JS. (2010) Copper homeostasis in Salmonella is atypical and copper-CueP is a major periplasmic metal complex. J. Biol. Chem. 285, 25259-25268.
  • Mastroeni P*, Grant AJ*, Restif O, Maskell DJ. (2009) A dynamic view of the spread and intracellular distribution of Salmonella enterica. Nat. Rev. Microbiol. 7, 73-80.
  • Wright JA, Grant AJ, Hurd D, Harrison M, Guccione E, Kelly DJ, Maskell DJ.  (2009) Metabolite and transcriptome analysis of Campylobacter jejuni in vitro growth dynamics reveals a stationary phase physiological switch. Microbiol. 155, 80-94.
  • Grant AJ*#, Foster GL*, McKinley TJ, Brown SP, Clare S, Maskell DJ, Mastroeni P. (2009) Bacterial growth rate and host factors as determinants of intracellular bacterial distributions in systemic Salmonella enterica infections. Infect. Immun. 77, 5608-5611.
  • Foster GL, Barr TA, Grant AJ, McKinley TJ, Bryant CE, MacDonald A, Gray D, Yamamoto M, Akira S, Maskell DJ and Mastroeni P. (2008) Virulent S. enterica infections can be exacerbated by concomitant infection of the host with a live attenuated S. enterica vaccine via Toll-like receptor 4-dependent interleukin-10 production with the involvement of both TRIF and MyD88. Immunol. 124, 469-479.
  • Grant AJ#, Sheppard M, Deardon R, Brown S, Foster G, Bryant C, Maskell DJ and Mastroeni P.  (2008) Caspase-3-dependent phagocyte death during systemic Salmonella enterica serovar Typhimurium infection of mice. Immunol.  125, 28-37.
  • Grant AJ#, Restif O, McKinley TJ, Sheppard M, Maskell DJ, and Mastroeni P.  (2008) Modelling within-host spatiotemporal dynamics of invasive bacterial disease. PLoS Biol. 6, e74.
  • Coward C, van Diemen PM, Conlan AJK, Grant AJ, Stevens MP, Gog JR, Jones MA, Maskell DJ. (2007) Competition between isogenic Campylobacter strains results in unpredictable population structures. Zoonoses Public Hlth. 54, 117-117.
  • Beech JS, Wheeler DW, Reckless J, Grant AJ, Price J, Mastroeni P, Grainger DJ and Menon DK. (2007) The MHP36 line of murine neural stem cells express functional CXCR1 chemokine receptors that initiate chemotaxis in vitro.  J. Neuroimmunol. 184, 198-208.
  • Conlan AJK, Coward C, Grant AJ, Maskell DJ and Gog J. (2007) Campylobacter jejuni colonisation and transmission in broiler chickens: A modelling perspective. J. R. Soc. Interface. 4, 819-829.
  • Coward C*, Grant AJ*#, Swift C, Philp J, Towler R, Heydarian M, Frost J and Maskell DJ.  (2006) Phase-variable surface structures are required for infection of Campylobacter jejuni by bacteriophages. Appl. Environ. Microbiol. 72, 4638-4647.
  • Grant AJ#, Woodward J and Maskell DJ.  (2006) Development of an ex vivo organ culture model using human gastro-intestinal tissue and Campylobacter jejuni. FEMS Microbiol. Lett. 263, 240-243.
  • Brown SP, Cornell SJ, Sheppard M, Grant AJ, Maskell DJ, Grenfell BT and Mastroeni P. (2006) Intracellular demography and the dynamics of S. typhimurium infection.  PLoS Biol. 4, 2091-2098.
  • Grant AJ*#, Coward C*, Jones MA, Woodall CA, Barrow PA and Maskell DJ.  (2005) Signature-tagged transposon mutagenesis studies demonstrate the dynamic nature of cecal colonization of 2-week-old chickens by Campylobacter jejuni. Appl. Environ. Microbiol. 71, 8031-8041
  • Grant AJ, Farris M, Alefounder P, Williams PH, Woodward MJ, O'Connor CD. (2003) Co-ordination of pathogenicity island expression by the BipA GTPase in enteropathogenic Escherichia coli (EPEC). Mol. Microbiol. 48, 507-521.
  • Owens RM, Grant A, Davies N, O'Connor CD. (2001) Copurification of the Lac repressor with polyhistidine-tagged proteins in immobilized metal affinity chromatography. Protein Expr. Purif. 21, 352-360.
  • Grant AJ, Haigh R, Williams P, O'Connor CD. (2001) An in vitro transposon system for highly regulated gene expression: construction of Escherichia coli strains with arabinose-dependent growth at low temperatures. Gene.  280, 145-151.
  • Farris M, Grant A, Richardson TB, O'Connor CD. (1998) BipA: a tyrosine-phosphorylated GTPase that mediates interactions between enteropathogenic Escherichia coli (EPEC) and epithelial cells. Mol. Microbiol. 28, 265-279
  • Farris M, Grant A, Jane S, Chad J, O'Connor CD. (1998) BipA affects Ca++ fluxes and phosphorylation of the translocated intimin receptor (Tir/Hp90) in host epithelial cells infected with enteropathogenic E. coli. Biochem. Soc. Trans. 26, S225.

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